Hotspot

petB

LSC · 76678-78084 · 1407 bp

Back to Chonemorpha

Pi 0.00356

MarkerSeek score 61.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1407
variable_sites: 5
pi: 0.00356
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTCGATCGCGGAATTTCCTT	CCTTCCCCAAGCGATAAATGA	45.0	47.6	58.0	58.1	1493-1497 bp	1.0	71.8
petB_p2	TTCGATCGCGGAATTTCCTT	TCCTTCCCCAAGCGATAAATG	45.0	47.6	58.0	58.1	1494-1498 bp	1.0	71.8
petB_p3	TTCGATCGCGGAATTTCCTT	TTCCTTCCCCAAGCGATAAA	45.0	45.0	58.0	56.8	1495-1499 bp	1.0	70.9
petB_p4	TTCGATCGCGGAATTTCCTT	ATTCCTTCCCCAAGCGATAA	45.0	45.0	58.0	56.6	1496-1500 bp	1.0	69.0
petB_p5	TTCGATCGCGGAATTTCCTT	TCCTTCCCCAAGCGATAAAT	45.0	45.0	58.0	56.6	1494-1498 bp	1.0	69.0