Hotspot

matK

LSC · 1940-3469 · 1530 bp

Back to Chonemorpha

Pi 0.00198

MarkerSeek score 60.60

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACGAGCTACTTACATGCTACA	TGTTTTGACTCTATCGCGCT	42.9	45.0	56.6	57.6	1697-1709 bp	1.0	58.4
matK_p2	ACGAGCTACTTACATGCTACA	CTGTTTTGACTCTATCGCGC	42.9	50.0	56.6	57.3	1698-1710 bp	1.0	55.7
matK_p3	ACGAGCTACTTACATGCTACAT	TGTTTTGACTCTATCGCGCT	40.9	45.0	57.1	57.6	1697-1709 bp	1.0	54.4
matK_p4	ACGAGCTACTTACATGCTACA	ACCCTGTTTTGACTCTATCGC	42.9	47.6	56.6	58.0	1701-1713 bp	1.0	53.1
matK_p5	GAGCTACTTACATGCTACATCGA	TGTTTTGACTCTATCGCGCT	43.5	45.0	57.9	57.6	1695-1707 bp	1.0	52.9