Hotspot

ndhG-ndhI

SSC · 120248-120797 · 550 bp

Back to Chonemorpha

Pi 0.0223

MarkerSeek score 72.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 550
variable_sites: 12
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCCTAGGCCTCCTAAGATC	TCGCACAATTTCGAATTCGC	60.0	45.0	58.1	58.1	666-669 bp	1.0	81.6
ndhG-ndhI_p2	AGGCCTCCTAAGATCAGACC	TCGCACAATTTCGAATTCGC	55.0	45.0	57.9	58.1	661-664 bp	1.0	81.3
ndhG-ndhI_p3	AGTAATACCACCCCTAGGCC	TCGCACAATTTCGAATTCGC	55.0	45.0	57.9	58.1	676-679 bp	1.0	81.1
ndhG-ndhI_p4	GTAATACCACCCCTAGGCCT	TCGCACAATTTCGAATTCGC	55.0	45.0	57.9	58.1	675-678 bp	1.0	81.1
ndhG-ndhI_p5	ATCAGACCCGATCCCAGAAA	TCGCACAATTTCGAATTCGC	50.0	45.0	58.1	58.1	649-652 bp	1.0	81.1