Hotspot

rpl14-rpl16

LSC · 82796-82939 · 144 bp

Back to Chonemorpha

Pi 0.014

MarkerSeek score 61.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 2
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCCGCTGTTATCTGCTACAT	AGCAGCATCCAAAATGCCTA	50.0	45.0	57.5	57.9	238 bp	1.0	75.6
rpl14-rpl16_p2	CCCGCTGTTATCTGCTACAT	TAGCAGCATCCAAAATGCCT	50.0	45.0	57.5	57.9	239 bp	1.0	75.6
rpl14-rpl16_p3	CCCGCTGTTATCTGCTACAT	ACTTTATGAAATGGGCGGGG	50.0	50.0	57.5	58.2	292 bp	1.0	74.8
rpl14-rpl16_p4	CCCGCTGTTATCTGCTACAT	CTTTATGAAATGGGCGGGGT	50.0	50.0	57.5	58.2	291 bp	1.0	74.8
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	TATGAAATGGGCGGGGTAAC	50.0	50.0	57.5	57.6	288 bp	1.0	73.8