Hotspot

petA-psbJ

LSC · 65106-65776 · 671 bp

Back to Chonemorpha

Pi 0.00298

MarkerSeek score 60.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 2
pi: 0.00298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	816 bp	1.0	77.0
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	813 bp	1.0	75.8
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	811 bp	1.0	74.5
petA-psbJ_p4	ACGTGTCCAAGGCCTTTTAT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	809 bp	1.0	72.6
petA-psbJ_p5	ATTACGTGTCCAAGGCCTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	812 bp	1.0	72.6