Hotspot

ndhC-trnV-UAC

LSC · 51945-53465 · 1521 bp

Back to Chonemorpha

Pi 0.0169
MarkerSeek score 71.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1521
variable_sites
23
pi
0.0169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGAGCTAAAACCCCGGAAAT AGAAGGTCTACGGTTCGAGA 50.0 50.0 57.9 57.5 1549-1652 bp 1.0 80.1
ndhC-trnV-UAC_p2 GGAGCTAAAACCCCGGAAAT GAAGGTCTACGGTTCGAGAC 50.0 55.0 57.9 57.5 1548-1651 bp 1.0 79.7
ndhC-trnV-UAC_p3 GGAGCTAAAACCCCGGAAAT GTTCGAGACCGTATAGCCCT 50.0 55.0 57.9 58.7 1537-1640 bp 1.0 78.6
ndhC-trnV-UAC_p4 GGAGCTAAAACCCCGGAAAT GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.9 57.2 1550-1653 bp 1.0 77.4
ndhC-trnV-UAC_p5 GGAGCTAAAACCCCGGAAAT CGAGAAGGTCTACGGTTCGA 50.0 55.0 57.9 58.9 1551-1654 bp 1.0 76.7