Hotspot

trnT-UGU-trnL-UAA

LSC · 47829-48618 · 790 bp

Back to Chonemorpha

Pi 0.0026

MarkerSeek score 62.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 2
pi: 0.0026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	840-851 bp	1.0	79.2
trnT-UGU-trnL-UAA_p2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	891-902 bp	1.0	78.3
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	872-883 bp	1.0	78.3
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	834-845 bp	1.0	77.9
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	885-896 bp	1.0	77.0