Hotspot

trnE-UUC-trnT-GGU

LSC · 31852-32766 · 915 bp

Back to Chonemorpha

Pi 0.00877
MarkerSeek score 64.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
915
variable_sites
8
pi
0.00877
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTCGTGATCAACTTCGACCC GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 1063-1097 bp 1.0 80.2
trnE-UUC-trnT-GGU_p2 AGAGAGATGTCCTGGACCAC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 1004-1038 bp 1.0 79.7
trnE-UUC-trnT-GGU_p3 GAGAGATGTCCTGGACCACT GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 1003-1037 bp 1.0 79.7
trnE-UUC-trnT-GGU_p4 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1019-1053 bp 1.0 79.4
trnE-UUC-trnT-GGU_p5 TTCGTGATCAACTTCGACCC TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.9 57.9 1028-1062 bp 1.0 79.4