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petN-psbM

LSC · 29304-30493 · 1190 bp

Back to Chonemorpha

Pi 0.00504

MarkerSeek score 61.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1190
variable_sites: 6
pi: 0.00504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TTTCCCTTTCGCTCGTAGTG	TCATTCTAGTTCCTACCGCCT	50.0	47.6	57.9	58.0	1296 bp	1.0	71.5
petN-psbM_p2	ACATTTTCCCTTTCGCTCGT	TCATTCTAGTTCCTACCGCCT	45.0	47.6	58.1	58.0	1300 bp	1.0	71.3
petN-psbM_p3	TTTCCCTTTCGCTCGTAGTG	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	57.9	57.9	1292 bp	1.0	71.0
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.2	58.0	1338 bp	1.0	70.9
petN-psbM_p5	ACATTTTCCCTTTCGCTCGT	TCTAGTTCCTACCGCCTTTCT	45.0	47.6	58.1	57.9	1296 bp	1.0	70.7