Hotspot

petF

Genome · 139831-140127 · 297 bp

Back to Chondracanthus

Pi 0.0976

MarkerSeek score 72.40

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: Genome
length_bp: 297
variable_sites: 29
pi: 0.0976
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	GGTGCAGGCGTAGTTTCTAA	ATGCCAGCACTTAGGAATGT	50.0	45.0	57.9	57.2	1209 bp	0.5	52.8
petF_p2	TAAAACTGTAGGTGCAGGCG	ATGCCAGCACTTAGGAATGT	50.0	45.0	58.2	57.2	1219 bp	0.5	51.9
petF_p3	GGTGCAGGCGTAGTTTCTAA	AGTGTTGAAGGTCTTCTGCA	50.0	45.0	57.9	57.0	1089 bp	0.5	51.2
petF_p4	GGTGCAGGCGTAGTTTCTAA	TGCCAGCACTTAGGAATGTAC	50.0	47.6	57.9	58.0	1208 bp	0.5	51.2
petF_p5	TAAAACTGTAGGTGCAGGCG	AGTGTTGAAGGTCTTCTGCA	50.0	45.0	58.2	57.0	1099 bp	0.5	50.4