Hotspot

moeB

Genome · 155748-156848 · 1101 bp

Back to Chondracanthus

Pi 0.0799

MarkerSeek score 76.30

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1101
variable_sites: 88
pi: 0.0799
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	GCATAACCAGGTGCTACTTT	GAAGGACTTGAACCCTCGAC	45.0	55.0	55.7	57.9	1465-1466 bp	1.0	67.3
moeB_p2	GCATAACCAGGTGCTACTTT	GTGTTGCTTTTCCCGCATTT	45.0	45.0	55.7	58.1	1748-1752 bp	1.0	67.2
moeB_p3	GCATAACCAGGTGCTACTTT	ATCACGCCTTGAAGGACTTG	45.0	50.0	55.7	58.2	1475-1476 bp	1.0	66.4
moeB_p4	GCATAACCAGGTGCTACTTT	TGAAGGACTTGAACCCTCGA	45.0	50.0	55.7	58.3	1466-1467 bp	1.0	65.6
moeB_p5	GCATAACCAGGTGCTACTTT	CTGAGGCTGGAGGAGTTTTC	45.0	55.0	55.7	57.9	1923 bp	0.5	40.9