Hotspot

cemA

Genome · 68577-69413 · 837 bp

Back to Chondracanthus

Pi 0.043

MarkerSeek score 69.50

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 837
variable_sites: 36
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TAGGCGCAATACGTATGCTC	TCCTCTAGATTGATACCATGCA	50.0	40.9	57.9	56.1	2277 bp	0.5	28.6
cemA_p2	GCCACAGATGCTTGTAGCTA	TCCTCTAGATTGATACCATGCA	50.0	40.9	57.7	56.1	1714 bp	0.5	26.6
cemA_p3	TAGGCGCAATACGTATGCTC	ATCCTCTAGATTGATACCATGCA	50.0	39.1	57.9	56.6	2278 bp	0.5	24.7
cemA_p4	TAGGCGCAATACGTATGCTC	TCCTCTAGATTGATACCATGCAT	50.0	39.1	57.9	56.6	2277 bp	0.5	24.7
cemA_p5	TAGGCGCAATACGTATGCTC	CCTCTAGATTGATACCATGCA	50.0	42.9	57.9	54.6	2276 bp	0.5	24.3