Hotspot

rpl2-rps19

Genome · 127414-127426 · 13 bp

Back to Chondracanthus

Pi 0.154

MarkerSeek score 77.60

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: Genome
length_bp: 13
variable_sites: 2
pi: 0.154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	ACACCATGGGGAAAAGCTAC	TGATTCGATATACGGGCCTT	50.0	45.0	57.8	56.2	139 bp	1.0	65.0
rpl2-rps19_p2	ACACCATGGGGAAAAGCTAC	TTGATTCGATATACGGGCCT	50.0	45.0	57.8	56.2	140 bp	1.0	65.0
rpl2-rps19_p3	ACACCATGGGGAAAAGCTAC	TGATTCGATATACGGGCCTTT	50.0	42.9	57.8	57.0	139 bp	1.0	63.1
rpl2-rps19_p4	ACACCATGGGGAAAAGCTAC	TTGATTCGATATACGGGCCTT	50.0	42.9	57.8	57.0	140 bp	1.0	63.1
rpl2-rps19_p5	ACACCATGGGGAAAAGCTAC	TTTGATTCGATATACGGGCCT	50.0	42.9	57.8	57.0	141 bp	1.0	63.1