Hotspot

ycf3-atpB

Genome · 96588-96937 · 350 bp

Back to Chondracanthus

Pi 0.127

MarkerSeek score 69.60

Primer available yes

Label ycf3-atpB

Feature Metadata

label_name: ycf3-atpB
feature_type: igs
region: Genome
length_bp: 350
variable_sites: 30
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-atpB_p1	GCAAGTAATGGAGAGCACGT	ACACCTGCTCCTCCAAAAAG	50.0	50.0	58.3	58.0	980-1093 bp	1.0	86.0
ycf3-atpB_p2	GCAAGTAATGGAGAGCACGT	CACCTGCTCCTCCAAAAAGT	50.0	50.0	58.3	58.0	979-1092 bp	1.0	86.0
ycf3-atpB_p3	TGCAAGTAATGGAGAGCACG	CACCTGCTCCTCCAAAAAGT	50.0	50.0	58.3	58.0	980-1093 bp	1.0	86.0
ycf3-atpB_p4	GCAAGTAATGGAGAGCACGT	CCTTCTCTAGTGCGTTCACC	50.0	55.0	58.3	58.0	1079-1192 bp	1.0	85.8
ycf3-atpB_p5	TGCAAGTAATGGAGAGCACG	CCTTCTCTAGTGCGTTCACC	50.0	55.0	58.3	58.0	1080-1193 bp	1.0	85.8