Hotspot

trnC(gca)-cbbX

Genome · 60514-60587 · 74 bp

Back to Chondracanthus

Pi 0.135
MarkerSeek score 77.00
Primer available yes
Label trnC(gca)-cbbX

Feature Metadata

label_name
trnC(gca)-cbbX
feature_type
igs
region
Genome
length_bp
74
variable_sites
10
pi
0.135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC(gca)-cbbX_p1 TTGCAATCCTCTGCCTTACC ACAATCAGAAGATATTCTAAAGAGT 50.0 28.0 57.9 53.8 153 bp 1.0 45.2
trnC(gca)-cbbX_p2 AGGATTTGCAATCCTCTGCC ACAATCAGAAGATATTCTAAAGAGT 50.0 28.0 58.2 53.8 158 bp 1.0 45.1
trnC(gca)-cbbX_p3 GGATTTGCAATCCTCTGCCT ACAATCAGAAGATATTCTAAAGAGT 50.0 28.0 58.2 53.8 157 bp 1.0 45.1
trnC(gca)-cbbX_p4 CGGTACCCAGATTCGAACTG ACAATCAGAAGATATTCTAAAGAGT 55.0 28.0 58.1 53.8 185 bp 1.0 44.3
trnC(gca)-cbbX_p5 GTTAGGCGGTACCCAGATTC ACAATCAGAAGATATTCTAAAGAGT 55.0 28.0 57.8 53.8 191 bp 1.0 44.2