Hotspot

psbF-psbL

Genome · 31794-31802 · 9 bp

Back to Chondracanthus

Pi 0.111
MarkerSeek score 76.70
Primer available yes
Label psbF-psbL

Feature Metadata

label_name
psbF-psbL
feature_type
igs
region
Genome
length_bp
9
variable_sites
1
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbL_p1 TCCATGGATTGGCTATTCCA ATTAGGATTAGGACCACTCA 45.0 40.0 56.3 52.4 101 bp 1.0 40.8
psbF-psbL_p2 TCCATGGATTGGCTATTCCA TTAGGATTAGGACCACTCAT 45.0 40.0 56.3 52.4 100 bp 1.0 40.8
psbF-psbL_p3 TCCATGGATTGGCTATTCCA ATTAGGATTAGGACCACTCAT 45.0 38.1 56.3 53.1 101 bp 1.0 40.8
psbF-psbL_p4 GTTAGCTATCCATGGATTGGCT ATTAGGATTAGGACCACTCA 45.5 40.0 58.0 52.4 109 bp 1.0 40.7
psbF-psbL_p5 GTTAGCTATCCATGGATTGGCT TTAGGATTAGGACCACTCAT 45.5 40.0 58.0 52.4 108 bp 1.0 40.7