Hotspot

rpoC2

Genome · 51427-53736 · 2310 bp

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Pi 0.23

MarkerSeek score 78.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 2310
variable_sites: 1011
pi: 0.23
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTGATCAGATGGCGGTTCAT	AATGGGGCCATTTTAGGGTG	50.0	50.0	58.0	58.1	2901 bp	0.166667	45.4
rpoC2_p2	TTGATGGTGATCAGATGGCG	AATGGGGCCATTTTAGGGTG	50.0	50.0	58.0	58.1	2907 bp	0.166667	45.4
rpoC2_p3	ACTTTACAGGGTGGTCAGGA	TGAGCTGCTTGAGGTTTTGT	50.0	45.0	57.9	57.9	2775 bp	0.166667	45.3
rpoC2_p4	TGTTTCTGGCCGTGCTATTT	AATGGGGCCATTTTAGGGTG	45.0	50.0	58.1	58.1	2966 bp	0.166667	44.9
rpoC2_p5	TTGTTTCTGGCCGTGCTATT	AATGGGGCCATTTTAGGGTG	45.0	50.0	58.1	58.1	2967 bp	0.166667	44.9