Hotspot

rpoB

Genome · 45918-49382 · 3465 bp

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Pi 0.2

MarkerSeek score 75.70

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: Genome
length_bp: 3465
variable_sites: 1396
pi: 0.2
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	AATGGGCGTTAGCTCTAAGC	AATGAAGTACTTCGACGGGC	50.0	50.0	58.0	58.0	771 bp	0.166667	46.3
rpoB_p2	AATGGGCGTTAGCTCTAAGC	GGCCTGAACGCCCTAATAAA	50.0	50.0	58.0	58.0	1097 bp	0.166667	45.9
rpoB_p3	AATGGGCGTTAGCTCTAAGC	AATTGGGTTTGTTTGGTGCG	50.0	45.0	58.0	58.1	736 bp	0.166667	45.9
rpoB_p4	AATGGGCGTTAGCTCTAAGC	CCCATTAAGGCCCTGTTACC	50.0	55.0	58.0	57.9	1224 bp	0.166667	45.8
rpoB_p5	ATCTTACCGCAACAAGGGAC	AATGAAGTACTTCGACGGGC	50.0	50.0	57.9	58.0	887 bp	0.166667	45.7