Hotspot

psbB

Genome · 31101-32639 · 1539 bp

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Pi 0.125

MarkerSeek score 80.10

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: Genome
length_bp: 1539
variable_sites: 420
pi: 0.125
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CCGCCTTACTGTCTTGTCTC	CCTTACCACTTGGCTACGAC	55.0	55.0	58.0	58.0	2744 bp	0.166667	46.6
psbB_p2	CCGCCTTACTGTCTTGTCTC	CTTACCACTTGGCTACGACC	55.0	55.0	58.0	58.0	2743 bp	0.166667	46.6
psbB_p3	CCGCCTTACTGTCTTGTCTC	ACCAGGGGCTACTTTACCAT	55.0	50.0	58.0	58.0	2447 bp	0.166667	46.5
psbB_p4	GTCCAGTGGCAAGTGTTGTA	CCTTACCACTTGGCTACGAC	50.0	55.0	58.0	58.0	2312 bp	0.166667	46.3
psbB_p5	GTCCAGTGGCAAGTGTTGTA	CTTACCACTTGGCTACGACC	50.0	55.0	58.0	58.0	2311 bp	0.166667	46.3