Hotspot

ycf12-rbcL

Genome · 22978-23081 · 104 bp

Back to Chloropicon

Pi 0.174

MarkerSeek score 79.20

Primer available yes

Label ycf12-rbcL

Feature Metadata

label_name: ycf12-rbcL
feature_type: igs
region: Genome
length_bp: 104
variable_sites: 39
pi: 0.174
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf12-rbcL_p1	AATATAGGGACGGTGAGCGA	CACCACGTAAGCACTCGTAA	50.0	50.0	58.0	57.9	920 bp	0.166667	46.2
ycf12-rbcL_p2	AATATAGGGACGGTGAGCGA	CCACGTAAGCACTCGTAACA	50.0	50.0	58.0	57.9	918 bp	0.166667	46.2
ycf12-rbcL_p3	AATATAGGGACGGTGAGCGA	AACAAGCACGACCGTAGTTT	50.0	45.0	58.0	58.1	902 bp	0.166667	46.1
ycf12-rbcL_p4	AATATAGGGACGGTGAGCGA	ACAAGCACGACCGTAGTTTT	50.0	45.0	58.0	58.1	901 bp	0.166667	46.1
ycf12-rbcL_p5	AATATAGGGACGGTGAGCGA	GTAACAAGCACGACCGTAGT	50.0	50.0	58.0	57.9	904 bp	0.166667	46.1