Hotspot

chlB-psbI

Genome · 6824-7615 · 792 bp

Back to Chloropicon

Pi 0.35

MarkerSeek score 80.40

Primer available yes

Label chlB-psbI

Feature Metadata

label_name: chlB-psbI
feature_type: igs
region: Genome
length_bp: 792
variable_sites: 519
pi: 0.35
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-psbI_p1	CAAGTGGACTCCAGATGCTC	ATTCTGGAGATGGGTGGTGA	55.0	50.0	58.0	58.0	1563 bp	0.166667	46.3
chlB-psbI_p2	CAAGTGGACTCCAGATGCTC	TCTGGAGATGGGTGGTGAAT	55.0	50.0	58.0	58.0	1561 bp	0.166667	46.3
chlB-psbI_p3	ATGTTGTTTGTGCGGGTACT	ATTCTGGAGATGGGTGGTGA	45.0	50.0	58.0	58.0	1957 bp	0.166667	46.2
chlB-psbI_p4	ATGTTGTTTGTGCGGGTACT	TCTGGAGATGGGTGGTGAAT	45.0	50.0	58.0	58.0	1955 bp	0.166667	46.2
chlB-psbI_p5	CAAGTGGACTCCAGATGCTC	GGGTTACGGCTTGGATCATT	55.0	50.0	58.0	57.9	1044 bp	0.166667	46.1