Hotspot

accD

LSC · 57767-59437 · 1671 bp

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Pi 0.081

MarkerSeek score 74.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1671
variable_sites: 106
pi: 0.081
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTATCCCGCAGTTTCAGACC	AAAGAAGCCATTGCAATCGC	55.0	45.0	58.1	58.0	2152-2520 bp	1.0	87.5
accD_p2	CTATCCCGCAGTTTCAGACC	CCGGGCCTTTTTAACCAGAT	55.0	50.0	58.1	58.2	2389-2757 bp	1.0	86.2
accD_p3	GGATCGTTTTCTATCCCGCA	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.8	58.0	2162-2530 bp	1.0	86.1
accD_p4	ACTCGGCTCAATCCTTTTCC	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	2736 bp	0.5	59.1
accD_p5	CTATCCCGCAGTTTCAGACC	TATTCGCCGGGCCTTTTTAA	55.0	45.0	58.1	57.9	2763 bp	0.5	58.5