Hotspot

ccsA-ndhD

SSC · 115204-115447 · 244 bp

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Pi 0.107
MarkerSeek score 78.40
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
244
variable_sites
25
pi
0.107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGTTATGGTTCATTCACATGA TGACAAGGTACAAGCTATCCT 33.3 42.9 52.8 56.1 307-308 bp 1.0 49.4
ccsA-ndhD_p2 AGTTATGGTTCATTCACATGA TGACAAGGTACAAGCTATCC 33.3 45.0 52.8 54.5 307-308 bp 1.0 49.4
ccsA-ndhD_p3 AGTTATGGTTCATTCACATGA GACAAGGTACAAGCTATCCT 33.3 45.0 52.8 54.2 306-307 bp 1.0 49.4
ccsA-ndhD_p4 AGTTATGGTTCATTCACATGA TTGACAAGGTACAAGCTATCCT 33.3 40.9 52.8 56.8 308-309 bp 1.0 49.3
ccsA-ndhD_p5 AGTTATGGTTCATTCACATGA TTGACAAGGTACAAGCTATCC 33.3 42.9 52.8 55.3 308-309 bp 1.0 49.3