Hotspot

rrn4.5-rrn5

IRb · 107593-107813 · 221 bp

Back to Chlorophytum

Pi 0.0505

MarkerSeek score 79.20

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 221
variable_sites: 11
pi: 0.0505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	335-338 bp	1.0	82.7
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	333-336 bp	1.0	82.7
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	331-334 bp	1.0	82.7
rrn4.5-rrn5_p4	GCTGAGGCATCCTAACAGAC	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.1	58.0	333-336 bp	1.0	82.6
rrn4.5-rrn5_p5	CAGCTGAGGCATCCTAACAG	TCCCCTACAGTATCGTCACC	55.0	55.0	58.0	57.9	343-346 bp	1.0	82.4