Hotspot

ycf4-cemA

LSC · 60769-61407 · 639 bp

Back to Chlorophytum

Pi 0.0341

MarkerSeek score 74.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 19
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.9	57.8	647-729 bp	1.0	84.3
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.1	57.8	649-731 bp	1.0	84.0
ycf4-cemA_p3	GGAACCATTCCCTTGACTCG	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.3	57.8	729-811 bp	1.0	82.6
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.7	57.8	652-734 bp	1.0	82.3
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.4	57.8	656-738 bp	1.0	82.1