Hotspot

trnS-GCU-trnG-UCC

LSC · 8060-9122 · 1063 bp

Back to Chlorophytum

Pi 0.0321

MarkerSeek score 72.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 34
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TGATCAGAGAGAGCGGAGAG	AAAGTTTTTGGGGACGGGAA	55.0	45.0	58.0	57.8	1264-1355 bp	1.0	80.8
trnS-GCU-trnG-UCC_p2	AAGTGATCAGAGAGAGCGGA	AAAGTTTTTGGGGACGGGAA	50.0	45.0	57.9	57.8	1267-1358 bp	1.0	79.9
trnS-GCU-trnG-UCC_p3	AATCTGCCGCTTTAGTCCAC	AAAGTTTTTGGGGACGGGAA	50.0	45.0	58.3	57.8	1197-1288 bp	1.0	79.1
trnS-GCU-trnG-UCC_p4	GATCAGAGAGAGCGGAGAGA	AAAGTTTTTGGGGACGGGAA	55.0	45.0	57.7	57.8	1263-1354 bp	1.0	79.0
trnS-GCU-trnG-UCC_p5	ATCAGAGAGAGCGGAGAGAG	AAAGTTTTTGGGGACGGGAA	55.0	45.0	57.7	57.8	1262-1353 bp	1.0	78.9