Hotspot

rps16-trnQ-UUG

LSC · 5572-6736 · 1165 bp

Back to Chlorophytum

Pi 0.0493

MarkerSeek score 80.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 56
pi: 0.0493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1221-1238 bp	1.0	83.7
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1232-1249 bp	1.0	83.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1225-1242 bp	1.0	79.0
rps16-trnQ-UUG_p4	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.2	58.1	1233-1250 bp	1.0	78.7
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1213-1230 bp	1.0	78.6