Hotspot

rps3

Genome · 12226-12894 · 669 bp

Back to Chloroparvula

Pi 0.272

MarkerSeek score 82.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 669
variable_sites: 176
pi: 0.272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CACATGTACGTGGAAGCGTA	GGTGAATAGGACGCGTTTCT	50.0	50.0	58.0	58.0	1714 bp	0.5	59.8
rps3_p2	TTGCACTTGGTCAACGTACA	GGTGAATAGGACGCGTTTCT	45.0	50.0	58.0	58.0	1609 bp	0.5	59.7
rps3_p3	GCACAATGGTCGTCAACATG	GGTGAATAGGACGCGTTTCT	50.0	50.0	58.0	58.0	1354 bp	0.5	59.7
rps3_p4	CCGAATTGCACTTGGTCAAC	GGTGAATAGGACGCGTTTCT	50.0	50.0	57.9	58.0	1614 bp	0.5	59.5
rps3_p5	CACATGTACGTGGAAGCGTA	CGGTAAGGGGCAAGAAGATC	50.0	55.0	58.0	58.0	1802 bp	0.5	59.5