Hotspot

rpoC1

Genome · 21277-23625 · 2349 bp

Back to Chloroparvula

Pi 0.348

MarkerSeek score 81.80

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2349
variable_sites: 776
pi: 0.348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AAAGGAGGTCAAAGGCTTGG	CTTGCTCAAAGCCTAGACGT	50.0	50.0	58.0	57.9	2856 bp	0.5	59.3
rpoC1_p2	AAAGGAGGTCAAAGGCTTGG	GTCACCAGCTCCAATGGAAA	50.0	50.0	58.0	58.1	2891 bp	0.5	59.3
rpoC1_p3	AAAGGAGGTCAAAGGCTTGG	TCACCAGCTCCAATGGAAAC	50.0	50.0	58.0	58.1	2890 bp	0.5	59.3
rpoC1_p4	AAAGGAGGTCAAAGGCTTGG	GTTTGCTCGTAGACCACCAT	50.0	50.0	58.0	57.9	2816 bp	0.5	59.2
rpoC1_p5	AAAGGAGGTCAAAGGCTTGG	TCACCAGCTGTCAATTGACC	50.0	50.0	58.0	58.1	2992 bp	0.5	59.2