Hotspot

rbcL

Genome · 51794-53221 · 1428 bp

Back to Chloroparvula

Pi 0.531

MarkerSeek score 80.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: Genome
length_bp: 1428
variable_sites: 622
pi: 0.531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GTGCATCTTGGTCAGTTTGC	TGGGTCAGTCAGAAGTAGCA	50.0	50.0	57.9	58.0	2153 bp	0.5	59.5
rbcL_p2	GTGCATCTTGGTCAGTTTGC	GGTCAGTCAGAAGTAGCACG	50.0	55.0	57.9	58.0	2151 bp	0.5	59.4
rbcL_p3	GTGCATCTTGGTCAGTTTGC	ATGCCACGATCACAACGTAA	50.0	45.0	57.9	57.9	2529 bp	0.5	58.9
rbcL_p4	GTGCATCTTGGTCAGTTTGC	TGCCACGATCACAACGTAAT	50.0	45.0	57.9	57.9	2528 bp	0.5	58.9
rbcL_p5	GTGCATCTTGGTCAGTTTGC	GCAAATCCCTCGAGGTTCTT	50.0	50.0	57.9	57.9	1972 bp	0.5	58.6