Hotspot

psbC

Genome · 6360-7745 · 1386 bp

Back to Chloroparvula

Pi 0.446

MarkerSeek score 79.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1386
variable_sites: 613
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TCAGGTTGGTTCTTTGCTCC	ATGGGGGCGGATTGTAAATC	50.0	50.0	58.0	58.0	2395 bp	0.5	59.8
psbC_p2	GGCGGTCTATGGACATTTGT	ATGGGGGCGGATTGTAAATC	50.0	50.0	58.0	58.0	2566 bp	0.5	59.7
psbC_p3	TGGTGCAACGGTAGAAAACA	ATGGGGGCGGATTGTAAATC	45.0	50.0	58.0	58.0	2246 bp	0.5	59.6
psbC_p4	TCAGGTTGGTTCTTTGCTCC	TTGAACCCCTAACCTACGGT	50.0	50.0	58.0	58.0	2199 bp	0.5	59.6
psbC_p5	CACTTTCCGTGCATTCAACC	ATGGGGGCGGATTGTAAATC	50.0	50.0	57.9	58.0	2198 bp	0.5	59.6