Hotspot

psbA

Genome · 8490-9551 · 1062 bp

Back to Chloroparvula

Pi 0.415

MarkerSeek score 79.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 436
pi: 0.415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATTTACAATCCGCCCCCAT	TCCTACAATTCGAGCAGCAC	50.0	50.0	58.0	58.0	1894 bp	0.5	59.9
psbA_p2	GATTTACAATCCGCCCCCAT	TTGGCTCCTACAATTCGAGC	50.0	50.0	58.0	58.0	1899 bp	0.5	59.8
psbA_p3	GATTTACAATCCGCCCCCAT	CGAGCAAAAGAAGCAACAGG	50.0	50.0	58.0	58.0	1977 bp	0.5	59.6
psbA_p4	GATTTACAATCCGCCCCCAT	TATATAATCGGCGGCATGGC	50.0	50.0	58.0	58.1	1519 bp	0.5	59.5
psbA_p5	GATTTACAATCCGCCCCCAT	TATTATTCACGCCGTGGTGG	50.0	50.0	58.0	58.1	1584 bp	0.5	59.5