Hotspot

chlL

Genome · 46649-47500 · 852 bp

Back to Chloroparvula

Pi 0.484

MarkerSeek score 79.60

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 852
variable_sites: 327
pi: 0.484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	TGCTTGTAGTAGGTGTCCCA	CGCCATACCTTCCAAATCCA	50.0	50.0	58.0	57.9	1783 bp	0.5	59.4
chlL_p2	TGCTTGTAGTAGGTGTCCCA	TGCAAGTTCCAATCCACACA	50.0	45.0	58.0	57.9	1745 bp	0.5	59.3
chlL_p3	TGCTTGTAGTAGGTGTCCCA	TGTGCAAGTTCCAATCCACA	50.0	45.0	58.0	57.9	1747 bp	0.5	59.3
chlL_p4	TGCTTGTAGTAGGTGTCCCA	TCGCCTTGTGTAAACGCATA	50.0	45.0	58.0	57.9	1863 bp	0.5	59.2
chlL_p5	TGCTTGTAGTAGGTGTCCCA	TCACTTGGGCTGAAATGTCC	50.0	50.0	58.0	58.1	1661 bp	0.5	59.1