Hotspot

rpl16

LSC · 77952-79464 · 1513 bp

Back to Chloris

Pi 0.0137

MarkerSeek score 53.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1513
variable_sites: 31
pi: 0.0137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCCTCGGCAATCG	TCAAAATTGCAGGCCGTCTA	50.0	45.0	58.1	57.8	2244-2291 bp	1.0	80.0
rpl16_p2	GGGGTACTGCGTCTTTGATT	TCAAAATTGCAGGCCGTCTA	50.0	45.0	57.9	57.8	2068-2115 bp	1.0	79.7
rpl16_p3	GCTCCACTGTTATCCGCTAC	TCAAAATTGCAGGCCGTCTA	55.0	45.0	58.1	57.8	1967-2014 bp	1.0	79.5
rpl16_p4	TCAGCAATAGTGTCCTTGCC	TCAAAATTGCAGGCCGTCTA	50.0	45.0	57.9	57.8	2454-2501 bp	1.0	79.4
rpl16_p5	CATTTGGGGTACTGCGTCTT	TCAAAATTGCAGGCCGTCTA	50.0	45.0	58.2	57.8	2073-2120 bp	1.0	79.2