Hotspot

rpl32-trnL-UAG

SSC · 105060-105508 · 449 bp

Back to Chloris

Pi 0.0476

MarkerSeek score 74.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 449
variable_sites: 29
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCAAAAGGTTTTTCTGGGT	AGCGTGTCTACCGATTTCAC	40.0	50.0	55.5	58.0	489-500 bp	1.0	66.7
rpl32-trnL-UAG_p2	ACCAAAAGGTTTTTCTGGGT	CAGCGTGTCTACCGATTTCA	40.0	50.0	55.5	58.0	490-501 bp	1.0	66.7
rpl32-trnL-UAG_p3	ACCAAAAGGTTTTTCTGGGT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	55.5	57.9	499-510 bp	1.0	65.6
rpl32-trnL-UAG_p4	ACCAAAAGGTTTTTCTGGGT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	55.5	57.8	497-508 bp	1.0	65.1
rpl32-trnL-UAG_p5	ACCAAAAGGTTTTTCTGGGT	GCTTGAGCACTGCTTCCTAA	40.0	50.0	55.5	58.2	513-524 bp	1.0	65.0