Hotspot

ndhF-rpl32

SSC · 104032-104894 · 863 bp

Back to Chloris

Pi 0.017

MarkerSeek score 58.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 863
variable_sites: 22
pi: 0.017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAGTCCCAACCCCATTGAC	TGACATCGAAGTACGTTTTT	50.0	35.0	58.0	53.2	975-989 bp	1.0	43.9
ndhF-rpl32_p2	AAAGTCCCAACCCCATTGAC	TTGACATCGAAGTACGTTTT	50.0	35.0	58.0	53.2	976-990 bp	1.0	43.9
ndhF-rpl32_p3	AAAGTCCCAACCCCATTGAC	TTTGACATCGAAGTACGTTT	50.0	35.0	58.0	53.2	977-991 bp	1.0	43.9
ndhF-rpl32_p4	AAAGTCCCAACCCCATTGAC	TTTTGACATCGAAGTACGTT	50.0	35.0	58.0	53.2	978-992 bp	1.0	43.9
ndhF-rpl32_p5	AAAGTCCCAACCCCATTGAC	TTTTTGACATCGAAGTACGT	50.0	35.0	58.0	53.2	979-993 bp	1.0	43.9