Hotspot

rpl14-rpl16

LSC · 77841-77951 · 111 bp

Back to Chloris

Pi 0.03

MarkerSeek score 63.80

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 5
pi: 0.03
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GCTCCACTGTTATCCGCTAC	AATGCCCATACGAAGCCAAT	55.0	45.0	58.1	57.9	198-231 bp	1.0	80.6
rpl14-rpl16_p2	GCTCCACTGTTATCCGCTAC	ATGCCCATACGAAGCCAATT	55.0	45.0	58.1	57.9	197-230 bp	1.0	80.6
rpl14-rpl16_p3	AGCTCCACTGTTATCCGCTA	AATGCCCATACGAAGCCAAT	50.0	45.0	58.2	57.9	199-232 bp	1.0	79.9
rpl14-rpl16_p4	AGCTCCACTGTTATCCGCTA	ATGCCCATACGAAGCCAATT	50.0	45.0	58.2	57.9	198-231 bp	1.0	79.9
rpl14-rpl16_p5	GCTCCACTGTTATCCGCTAC	TGCCCATACGAAGCCAATTT	55.0	45.0	58.1	58.2	196-229 bp	1.0	79.9