Hotspot

petG-trnW-CCA

LSC · 64162-64287 · 126 bp

Back to Chloris

Pi 0.0159
MarkerSeek score 64.40
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
126
variable_sites
3
pi
0.0159
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 AGCCGGATTATTCGTGACTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 224-226 bp 1.0 79.3
petG-trnW-CCA_p2 GCGTATTTGCAATACAGGCG CAAAACCCGATGTCGTAGGT 50.0 50.0 58.3 57.9 205-207 bp 1.0 79.2
petG-trnW-CCA_p3 CGGATTATTCGTGACTGCGT CAAAACCCGATGTCGTAGGT 50.0 50.0 58.4 57.9 221-223 bp 1.0 77.5
petG-trnW-CCA_p4 TCGTGACTGCGTATTTGCAA CAAAACCCGATGTCGTAGGT 45.0 50.0 58.5 57.9 213-215 bp 1.0 77.2
petG-trnW-CCA_p5 TTCGTGACTGCGTATTTGCA CAAAACCCGATGTCGTAGGT 45.0 50.0 58.5 57.9 214-216 bp 1.0 77.2