Hotspot

atpB-rbcL

LSC · 54507-55316 · 810 bp

Back to Chloris

Pi 0.00494

MarkerSeek score 64.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 6
pi: 0.00494
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGATCAATACGCCCTGCA	GCTTGAAATCCAACACCTGC	45.0	50.0	57.9	57.9	915-931 bp	1.0	80.4
atpB-rbcL_p2	TTTGATCAATACGCCCTGCA	TTCGTATTCCGGGGTGTAGT	45.0	50.0	57.9	58.1	961-977 bp	1.0	79.7
atpB-rbcL_p3	TATCCAGCACGGGTCCAATA	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	936-952 bp	1.0	79.7
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	933-949 bp	1.0	79.4
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	GCTTGAAATCCAACACCTGC	55.0	50.0	57.7	57.9	951-967 bp	1.0	79.1