Hotspot

trnR-UCU-trnfM-CAU

LSC · 36934-37104 · 171 bp

Back to Chloris

Pi 0.0572
MarkerSeek score 78.40
Primer available yes
Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name
trnR-UCU-trnfM-CAU
feature_type
igs
region
LSC
length_bp
171
variable_sites
14
pi
0.0572
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnfM-CAU_p1 AGAAGACCTCTGTCCTATCCA GGTAGCGCACAAAGCTCATA 47.6 50.0 56.9 58.3 244-249 bp 1.0 67.6
trnR-UCU-trnfM-CAU_p2 AGGTTTAGAAGACCTCTGTCCT GGTAGCGCACAAAGCTCATA 45.5 50.0 57.9 58.3 250-255 bp 1.0 66.8
trnR-UCU-trnfM-CAU_p3 AGAAGACCTCTGTCCTATCCA CCTTGGAGTTACGGGTTCAA 47.6 50.0 56.9 57.4 223-228 bp 1.0 66.6
trnR-UCU-trnfM-CAU_p4 AGAAGACCTCTGTCCTATCCA AACCTTGGAGTTACGGGTTC 47.6 50.0 56.9 57.4 225-230 bp 1.0 66.5
trnR-UCU-trnfM-CAU_p5 AGAAGACCTCTGTCCTATCCA ACCTTGGAGTTACGGGTTCA 47.6 50.0 56.9 58.6 224-229 bp 1.0 66.4