Hotspot

atpI-atpH

LSC · 32230-33043 · 814 bp

Back to Chloris

Pi 0.0148

MarkerSeek score 69.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 18
pi: 0.0148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CTCTACAGCTTGTCCTGCAG	50.0	55.0	57.5	58.0	969-970 bp	1.0	77.7
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CTCTACAGCTTGTCCTGCAG	45.0	55.0	58.6	58.0	982-983 bp	1.0	77.2
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	906-907 bp	1.0	76.7
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	950-951 bp	1.0	76.3
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	883-884 bp	1.0	76.3