Hotspot

trnC-GCA-rpoB

LSC · 18971-20063 · 1093 bp

Back to Chloris

Pi 0.0155

MarkerSeek score 61.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1093
variable_sites: 24
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1134-1177 bp	1.0	82.5
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1133-1176 bp	1.0	82.5
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1117-1160 bp	1.0	81.9
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.1	57.6	1118-1161 bp	1.0	81.6
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TCCGGGTATTGTGGACATTC	50.0	50.0	58.1	57.3	1135-1178 bp	1.0	79.5