Hotspot

trnD-GUC-psbM

LSC · 16133-17200 · 1068 bp

Back to Chloris

Pi 0.0188

MarkerSeek score 64.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 24
pi: 0.0188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.6	56.7	777-1232 bp	1.0	71.0
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	810-1265 bp	1.0	70.1
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.6	56.5	779-1234 bp	1.0	69.1
trnD-GUC-psbM_p4	TCAATTGGTTAGAGCACCGC	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.6	56.5	778-1233 bp	1.0	69.1
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.6	57.4	777-1232 bp	1.0	68.9