Hotspot

trnS-GCU-psbD

LSC · 8233-9108 · 876 bp

Back to Chloris

Pi 0.0141

MarkerSeek score 65.80

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 18
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1019-1093 bp	1.0	79.9
trnS-GCU-psbD_p2	GAAAGTCCCAAACGATCCGA	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1092-1166 bp	1.0	79.4
trnS-GCU-psbD_p3	TCGAAAGTCCCAAACGATCC	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1094-1168 bp	1.0	79.4
trnS-GCU-psbD_p4	AAAGTCCCAAACGATCCGAG	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1091-1165 bp	1.0	79.4
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1006-1080 bp	1.0	79.4