Hotspot

rps16-trnQ-UUG

LSC · 5623-6893 · 1271 bp

Back to Chloris

Pi 0.0191

MarkerSeek score 62.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1271
variable_sites: 35
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1339-1345 bp	1.0	81.3
rps16-trnQ-UUG_p2	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1350-1356 bp	1.0	79.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1343-1349 bp	1.0	76.6
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1331-1337 bp	1.0	76.1
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	1338-1344 bp	1.0	75.5