Hotspot

matK-trnK-UUU

LSC · 3229-3918 · 690 bp

Back to Chloris

Pi 0.0193

MarkerSeek score 64.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 20
pi: 0.0193
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CATAATCGTGGGCAAATGCA	TGGGTTGCTAACTCAATGGT	45.0	45.0	57.1	57.0	817-827 bp	1.0	68.4
matK-trnK-UUU_p2	ACATAATCGTGGGCAAATGC	TGGGTTGCTAACTCAATGGT	45.0	45.0	57.1	57.0	818-828 bp	1.0	68.4
matK-trnK-UUU_p3	CATAATCGTGGGCAAATGCA	TCTGGGTTGCTAACTCAATGG	45.0	47.6	57.1	57.9	819-829 bp	1.0	67.6
matK-trnK-UUU_p4	ACATAATCGTGGGCAAATGC	TCTGGGTTGCTAACTCAATGG	45.0	47.6	57.1	57.9	820-830 bp	1.0	67.6
matK-trnK-UUU_p5	CATAATCGTGGGCAAATGCA	CTGGGTTGCTAACTCAATGGT	45.0	47.6	57.1	58.2	818-828 bp	1.0	66.5