Hotspot

ndhF

SSC · 129443-131662 · 2220 bp

Back to Chloranthus

Pi 0.0159

MarkerSeek score 49.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 82
pi: 0.0159
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAATCAAGCCCCGTTTTTGG	TTTCACACATGCCCAAGTGA	50.0	45.0	57.9	57.9	2723-2767 bp	1.0	83.2
ndhF_p2	GAATCAAGCCCCGTTTTTGG	GTGCACGACAAAACCTGAAC	50.0	50.0	57.9	58.2	2625-2669 bp	1.0	82.4
ndhF_p3	GAATCAAGCCCCGTTTTTGG	ACCTGAACCTGAAGACCTGT	50.0	50.0	57.9	58.2	2613-2657 bp	1.0	82.3
ndhF_p4	TCAGAATCAAGCCCCGTTTT	TTTCACACATGCCCAAGTGA	45.0	45.0	57.7	57.9	2726-2770 bp	1.0	81.5
ndhF_p5	GAATCAAGCCCCGTTTTTGG	TTCACACATGCCCAAGTGAT	50.0	45.0	57.9	57.7	2722-2766 bp	1.0	81.4