Hotspot

clpP

LSC · 72743-74820 · 2078 bp

Back to Chloranthus

Pi 0.0137

MarkerSeek score 56.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2078
variable_sites: 63
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGTTCGAATGGAATCACGGA	ATGCTATGGACCTACCCCTC	50.0	55.0	58.0	58.0	2441-2468 bp	1.0	83.5
clpP_p2	GGTTCGAATGGAATCACGGA	ATACTCCAACCACCCCATGA	50.0	50.0	58.0	58.0	2737-2764 bp	1.0	83.4
clpP_p3	GGTTCGAATGGAATCACGGA	TGATACTCCAACCACCCCAT	50.0	50.0	58.0	58.0	2739-2766 bp	1.0	83.4
clpP_p4	CTGAACGAGTCGCACATACA	ATGCTATGGACCTACCCCTC	50.0	55.0	58.0	58.0	2700-2727 bp	1.0	83.2
clpP_p5	TGACCCTGTTAGTCCGTTCT	ATGCTATGGACCTACCCCTC	50.0	55.0	58.0	58.0	2957-2983 bp	0.714286	70.6