Hotspot

ccsA-trnL-UAG

SSC · 126998-127098 · 101 bp

Back to Chloranthus

Pi 0.0514

MarkerSeek score 65.90

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 101
variable_sites: 10
pi: 0.0514
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	TGATCGAAAAACAGATGTGAGT	GTGAAATCGGTAGACACGCT	36.4	50.0	56.0	58.0	224-229 bp	1.0	51.8
ccsA-trnL-UAG_p2	TGATCGAAAAACAGATGTGAGT	TGAAATCGGTAGACACGCTG	36.4	50.0	56.0	58.0	223-228 bp	1.0	51.8
ccsA-trnL-UAG_p3	TGATCGAAAAACAGATGTGAGT	TAGACACGCTGCTCTTAGGA	36.4	50.0	56.0	57.9	214-219 bp	1.0	51.0
ccsA-trnL-UAG_p4	TGATCGAAAAACAGATGTGAGT	CGACGATTGAACATGCATGG	36.4	50.0	56.0	57.9	273-278 bp	1.0	50.5
ccsA-trnL-UAG_p5	TGATCGAAAAACAGATGTGAGT	GTAGACACGCTGCTCTTAGG	36.4	55.0	56.0	57.8	215-220 bp	1.0	50.4