Hotspot

rpl16-rps3

LSC · 85495-85649 · 155 bp

Back to Chloranthus

Pi 0.0357

MarkerSeek score 66.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 15
pi: 0.0357
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CACTGGTTCCTTGGATCTCG	GGGTCAGAGAAGGTAGGGTT	55.0	55.0	58.0	58.0	417-445 bp	1.0	86.9
rpl16-rps3_p2	CACTGGTTCCTTGGATCTCG	TCGAATGGGTCAGAGAAGGT	55.0	50.0	58.0	58.1	423-451 bp	1.0	86.7
rpl16-rps3_p3	ACTGGTTCCTTGGATCTCGA	GGGTCAGAGAAGGTAGGGTT	50.0	55.0	58.1	58.0	416-444 bp	1.0	86.5
rpl16-rps3_p4	CACTGGTTCCTTGGATCTCG	GTCCAACACGAGGAATCGAA	55.0	50.0	58.0	57.9	690-718 bp	1.0	84.7
rpl16-rps3_p5	CACTGGTTCCTTGGATCTCG	TTCCAGAGGTCTCCAGGAAG	55.0	55.0	58.0	58.1	869-897 bp	1.0	84.3